Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Dec 12;2:e01020. doi: 10.7554/eLife.01020

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2013, Daley et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

PMC Copyright notice

Figure 9. — (A) Representative CD44 expression on unstimulated CD4SP thymocytes from wildtype, Rasgrp1^Anaef and Rasgrp1^−/− mice. (B) Mean relative CD44 from 22 Rasgrp1^Anaef, 13 wildtype, and 3 Rasgrp1^−/− mice compiled from seven experiments. In each experiment, the mean CD44 expression for the wildtype group was taken to be one, and values for individual mice were normalized to this. Unpaired Student’s t tests were used to compare Rasgrp1^Anaef and Rasgrp1^−/− with the wildtype group. **p<0.005, ***p<0.0005. (C) CD44 MFI on CD62L⁺Foxp3⁻CD4⁺ splenocytes from mixed bone marrow chimeras described in Figure 7F. Statistical comparisons used paired t tests within, and unpaired t tests between, groups of chimeras (n = 9 for both groups); ***p<0.001, **p<0.01. (D) CD44 MFI on TCR3A9+ (clonotype positive) CD4⁺Foxp3⁻ splenocytes from B10.BR mixed bone marrow chimeras containing CD45.1⁺ wildtype TCR3A9 plus either CD45.2⁺ wildtype (n = 8) or CD45.2⁺ Rasgrp1^Anaef (n = 7) 3A9 TCR-transgenic hematopoietic cells. Statistical comparisons in (C) and (D) used paired t tests within groups of chimeras and unpaired t tests between groups of chimeras. p value symbols: ***p<0.001, **p<0.01, *p<0.05. (E) Rasgrp1^Anaef increases CD44 expression levels but does not affect CD69 or CD5 expression. CD4SP thymocytes are analyzed from irradiated mice reconstituted with non-transgenic CD45.1⁺ wildtype mixed with CD45.2+ Rasgrp1^Anaef bone marrow.

DOI: http://dx.doi.org/10.7554/eLife.01020.017

Figure 9—figure supplement 1. — (A) Representative CD44 expression on unstimulated CD4SP thymocytes from wildtype, Rasgrp1^Anaef and Rasgrp1^−/− mice. (B) Mean relative CD44 from 22 Rasgrp1^Anaef, 13 wildtype, and 3 Rasgrp1^−/− mice compiled from seven experiments. In each experiment, the mean CD44 expression for the wildtype group was taken to be one, and values for individual mice were normalized to this. Unpaired Student’s t tests were used to compare Rasgrp1^Anaef and Rasgrp1^−/− with the wildtype group. **p<0.005, ***p<0.0005. (C) CD44 MFI on CD62L⁺Foxp3⁻CD4⁺ splenocytes from mixed bone marrow chimeras described in Figure 7F. Statistical comparisons used paired t tests within, and unpaired t tests between, groups of chimeras (n = 9 for both groups); ***p<0.001, **p<0.01. (D) CD44 MFI on TCR3A9+ (clonotype positive) CD4⁺Foxp3⁻ splenocytes from B10.BR mixed bone marrow chimeras containing CD45.1⁺ wildtype TCR3A9 plus either CD45.2⁺ wildtype (n = 8) or CD45.2⁺ Rasgrp1^Anaef (n = 7) 3A9 TCR-transgenic hematopoietic cells. Statistical comparisons in (C) and (D) used paired t tests within groups of chimeras and unpaired t tests between groups of chimeras. p value symbols: ***p<0.001, **p<0.01, *p<0.05. (E) Rasgrp1^Anaef increases CD44 expression levels but does not affect CD69 or CD5 expression. CD4SP thymocytes are analyzed from irradiated mice reconstituted with non-transgenic CD45.1⁺ wildtype mixed with CD45.2+ Rasgrp1^Anaef bone marrow.

DOI: http://dx.doi.org/10.7554/eLife.01020.017