Table 1.
Mutation
|
In vitro MMR (This work) | Pathologyc,d
|
Referencesd | |||
---|---|---|---|---|---|---|
Proteina | DNAb | MSI | IHC
|
|||
PMS2 | MLH1 | |||||
I18V | c.52A>G | + | 1, 2 | |||
R20Q | c.59G>A | + | H3 | 1, 2, 3, 4, 5 | ||
E41A | c.122A>C | - | 5 | |||
S46I | c.137G>T | - | H3, 6 | Neg3, 6 | Pos6 | 2, 3, 4, 6, 7, 8 |
S46N | c.137G>A | - | 7, 8 | |||
D60E | c.180C>G | + | LOVD | |||
Q205P | c.614A>C | + | LOVD | |||
G207E | c.620G>A | + | H9 | Neg9 | 9 | |
L263V | c.787C>G | + | LOVD | |||
A423T | c.1267G>A | + | 10 | |||
H479Q | c.1437C>G | + | 11, 12 | |||
T485K | c.1454C>A | + | H6 | Neg6 | Pos6 | 1, 2, 4, 6, 11 |
T511A | c.1531A>G | + | H3 | 2, 3, 6, 11, 12 | ||
T511M | c.1532C>T | + | LOVD | |||
Y519C | c.1556A>G | + | LOVD | |||
E541K | c.1621A>G | + | 1, 2, 5, 6 | |||
R563L | c.1688G>T | + | 1, 2, 8 | |||
L571I | c.1711C>A | + | 2 | |||
L585I | c.1753C>A | + | 1 | |||
T597S | c.1789A>T | + | L3 | 1, 3, 4, 5, 11 | ||
M622I | c.1866G>A | + | 1, 2, 4, 6, 8, 11 | |||
E663A | c.1988A>C | + | 8 | |||
E705K | c.2113G>A | - | 2, 6, 8, 13, 14 | |||
G750D | c.2249G>A | + | 8 | |||
M797R | c.2390T>G | + | 2 | |||
C843Y | c.2528G>A | - | 8 | |||
G857A | c.2570G>C | + | 1, 2, 6, 12 |
Amino acid numbering is based on the PMS2 reference sequence NP_000526.1 with +1 corresponding to the translation initiation amino acid.
Nucleotide numbering reflects cDNA numbering with +1 corresponding to the A of the translation initiation codon of the PMS2 GenBank reference sequence (NM_000535.5).
MSI=Microsatellite Instability. H=High, L=Low, IHC=Immunohistochemistry. Neg = Negative for staining, Pos = Positive for staining. In case cells are empty, the variant was not tested.
Variants have been selected from the LOVD (http://chromium.liacs.nl/LOVD2/colon_cancer/variants.php?select_db=PMS2&action=view_all&view=Prot_sub). Appropriate references are shown. References: 1 [Hendriks et al., 2006], 2 [Clendenning et al., 2006], 3 [Pastrello et al., 2011], 4 [Thompson et al., 2012], 5 [BorrĂ s et al., 2013], 6 [Nakagawa, 2004], 7 [Jackson et al., 2008], 8 [Senter et al., 2008], 9 [Montazer Haghighi et al., 2009], 10 [Ganster et al., 2010], 11 [Wang et al., 1999], 12 [Basil et al., 1999], 13 [DeschĂȘnes et al., 2007], 14 [van Oers et al., 2010].