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. Author manuscript; available in PMC: 2013 Dec 11.
Published in final edited form as: Cell Rep. 2013 Oct 31;5(3):10.1016/j.celrep.2013.09.046. doi: 10.1016/j.celrep.2013.09.046

Figure 5.

Figure 5

Xbp1 is not required for Spacemaker/Eyes shut secretion or Rh1 delivery into the rhabdomere. (A) Schematic of Xbp1 genomic region with the localization of P[lacW]Xbp1K13803, P[PTT-GB]Xbp1CB02061and P[SUPor-P]CG9418KG05183. Excisions 81 and 79 delete the DNA binding domain of Xbp1 and were generated from jumps of P[PTT-GB]Xbp1CB02061. In excision 81, the sequence from 4bp to 622bp downstream from Xbp1 start codon (ATG) is deleted. In excision 79, the sequence between 260bp upstream and 748bp downstream from Xbp1 start codon (ATG) is deleted. In excision 250, the open reading frame of Xbp1 is deleted from 5bp after the start codon, together with CG9418 and CG15657, until 2R:17036107, in the inter-genic region between CG15657 and CG30389. Excision 250 was generated from a jump of P[SUPor-P]CG9418KG05183. (B) By 62 h pupation, eyeless-Flipase induced clones of cells homozygous for Excision 79, labeled by the absence of myrGFP (green), have normal levels of Spam/Eys (blue) in the IRS. The rhabdomeres are stained with actin (red). (C) At 72 h. pupa, Rh1 (blue) is delivered to the rhabdomere in photoreceptors homozygous for Excision 79, labeled by the absence of myrRFP (red), but Xbp1 mutant photoreceptors have lower levels of Rh1 in the rhabdomere in than wild type (monochrome magnifications with wild type and mutant ommatidia). (D) At 82 h. pupa, photoreceptors homozygous for Excision 79 present higher levels of Rh1 in the rhabdomere than wild-type. Excision 79 homozygous photoreceptors show even distribution of Rh1 in the rhabdomere, while wild-type show the typical crescent-shaped gradient of concentration with higher levels at the rhabdomere base (monochrome magnifications). (E) Quantification of Rh1 intensity. Average of the ratio of Rh1 fluorescence intensity divided by respective area between pairs of adjacent Excision 79 homozygous and wild-type ommatidia, at 72 and 82 hours of pupal development (FluoEx79/AreaEx79/FluoWT/AreaWT). Around 70 pairs of Excision 79 homozygous and wild-type ommatidia were quantified at each time point. At 72 h. pupa, Excision 79 homozygous photoreceptors have reduced levels of (F) BiP/GRP78 (blue and monochrome) and (G) ninaA (blue and monochrome) but not (H) Cnx (blue and monochrome). Scale bars, 10 μm.