Table 1.

PCR primers used in the study

Gene	MGI Symbol	Forward primer	Reverse primer
TNF	Tnf	TTGGAGTCATTGCTCTGTGAA	GGGTCAGAGTAAAGGGGTCAG
MCP-1	Ccl2	AGGTGTCCCAAAGAAGCTGTA	ATGTCTGGACCCATTCCTTCT
IL-6	Il6	ATGGATGCTACCAAACTGGAT	TGAAGGACTCTGGCTTTGTCT
IL-1β	Il1b	CTCTCCACCTCAATGGACAGA	TTTTGTCGTTGCTTGGTTCTC
ICAM-1	Icam1	GCCTTGGTAGAGGTGACTGAG	GACCGGAGCTGAAAAGTTGTA
ELAM-1	Sele	CTCACTCCTGACATCGTCCTC	ACGTTGTAAGAAGGCACATGG
TGF-β1	Tgfb1	GAGAGCCCTGGATACCAACTA	GACAGAAGTTGGCATGGTAGC
TGF-β2	Tgfb2	TGGCCGAGCAGCGGATTGAAC	TGCACAGCGTCTGTCACGTCG
TGF-β3	Tgfb3	TGCTTCCGCAACCTGGAGGAGA	CTGCGCTGCGGAGGTATGGG
RANTES	Ccl5	ATATGGCTCGGACACCACTC	GTGACAAACACGACTGCAAGA
SLPI	Slpi	GTGACGGCAAATACAAGTGCT	CTGGGAGCAGGGAAGTAGTTT
INOS	Nos2	CAGCTGGGCTGTACAAACCTT	CATTGGAAGTGAAGCGTTTCG
NOX2	Cybb	CCAACTGGGATAACGAGTTCA	GAGAGTTTCAGCCAAGGCTTC
COX-2	COX2	TGGTGCCTGGTCTGATGATG	GTGGTAACCGCTCAGGTGTTG
MMP-2	Mmp2	GAATGCCATCCCTGATAACCT	CAGCCAGTCTGATTTGATGCT
MMP-9	Mmp9	ATTCGCGTGGATAAGGAGTTC	CCTTGTTCACCTCATTTTGGA
CLDN5	Cldn5	TGGTGGCACTCTTTGTTACCT	AGTGCTACCCGTGCCTTAACT
VE-CADHERIN	Cdh5	CTGTGGAGACCATAGGTGACAG	CTGGGATCTTTCTGGTGAGTG
PDGFB	Pdgfb	CGGCCGGTCCAGGTGAGAAAG	CACTTGCAGGCGAGGTGGTCC
PDGFRα	Pdgfra	CTCCTCACGCTGTGTGCGACC	GGCGGGATCCATCCAAGCCAT
PDGFRβ	Pdgfrb	ACTGCCGTGCAGCCCAATGAG	GGTGGAACTGGCAAGGCTGGG
VEGFA	Vegfa	CCAGGAGGACCTTGTGTGAT	GAGGAAGGGTAAGCCACTCAC
VEGFR1	Ft2	AGGAAGACGGTTCCTTCTGAG	CCCAGTGCACCTAGGTATGTC
VEGFR2	Kdr	GGGGAACTGAAGACAGGCTAC	CGGCCAAGAGGTTTTCCTAGT
ANG1	Angpt1	GCTTCCCACCACATCTTTCTT	CCTGTAATGTCGGCACATACC
ANG2	Angpt2	ACTGGGAAGGCAACGAGGCG	GCGGTCCCCGTGAGTCCTGTAA
TIE2	Tek	GGAAAATTTAACCCCATCTGC	CAGTGAATATGGCCACTGAGAA
tPA	Plat	AATAAAACCGTCACGAACAACA	TTTATTGATCATGCACACCAGAG
AQP4	Aqp4	TACATGGAGGTGGAGGACAAC	TCTTCTCTTCTCCACGGTCAA
HPRT	Hprt	CCGAGGATTTGGAAAAAGTGT	GGAAAATACAGCCAACACTGC

SLPI, secretory leukocyte protease inhibitor; COX-2, cyclooxygenase-2; CLDN5, claudin-5; MGI, mouse gene identifiers.