Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Adherent and Non-adherent dental pulp stem cells culture conditions. Human dental pulp cells were isolated and cultured in basal medium supplemented with 10% FCS (Day 0). After 12h, medium was changed for a basal medium supplemented with N2, EGF, bFGF and heparin (defined medium), and floating cells were isolated. Gene expressions in ADH-DPSCs, spheroids and spheroid-derived cells were analyzed by qPCR. Flow cytometry was performed on ADH-DPSCs after the second passage (P2).
