Table 1.
Phosphosites that exhibit increased phosphorylation after GH treatment
| Gene | Phosphosite2 | No. of Sites | Ratio (+GH/−GH) (5a/5b/15 min)3 | Function of phosphosite |
|---|---|---|---|---|
| 6330577E15Ri | [T70 = S71] | 1 | −/−/1.5 | Unknown |
| Acin1 | S710 | 1 | −/1.8/− | Unknown |
| Acly | S455 | 1 | 1.5/−/− | Increases catalytic activity (67) |
| Ahnak | S136 | 1 | 3.4/4.5/5.2 | Unknown |
| Ahnak | S217 | 1 | −/−/2.4 | Unknown |
| Ahnak | S4890 | 1 | 2.2/2.5/3.7 | Unknown |
| Ahnak | [S5557 >S5566 = T5567] | 1 | 2.1/−/− | Unknown |
| Ahnak | [T5569 >T5567 >T5571 >S5555 >S5566] | 2 | 2.5/−/− | Unknown |
| Ahnak2 | S652 | 1 | −/2.4/− | Unknown |
| Akap1 | S109 - isoforms 1,3,5,6 (S142 - isoforms 2,4) | 1 | 1.5/−/− | Unknown |
| Akap2 | S740 (S984) | 1 | −/1.9/1.6 | Unknown |
| Akap2 | [T738 >S740] [(T982 >S984)] | 1 | −/2.2/− | Unknown |
| Akt1s1 (PRAS40) | S184 (S255) | 1 | −/2.2/3.9 | Regulates PRAS40 interaction with raptor (87) |
| Akt1s1 (PRAS40) | T247 (T318) | 1 | 3.8/−/− | Suppresses PRAS40 inhibition of mTORC1 (60) |
| Arfgap2 | S145 | 1 | −/1.7/− | Unknown |
| Atp6v0a2 | S695 | 1 | 1.4/−/− | Unknown |
| Bat2 | S761 | 1 | −/1.5/− | Unknown |
| Bckdha | S334 (S338) | 1 | 1.6/−/− | Unknown |
| Bcl9l | [S116 >S118] | 1 | −/2.8/− | Unknown |
| Bcl9l | S118 | 1 | −/2.4/− | Unknown |
| Bclaf1 | S383 | 1 | 1.6/−/− | Unknown |
| Cad | S1859 | 1 | −/1.9/− | Unknown |
| Cast | S219 | 1 | −/1.5/− | Unknown |
| Cbx5 | S95 (S93) | 1 | −/2/− | Unknown |
| Cd2ap | S458 | 1 | −/1.5/− | Unknown |
| Cic | [S2280 >T2283 = S2284 >S2275 = T2277] | 2 | 1.4/−/− | Unknown |
| D930048N14Rik | T15 | 1 | −/4.1/− | Unknown |
| Dact3 | S409 | 1 | 2.2/−/− | Unknown |
| Dap | T56 | 1 | −/22/− | Unknown |
| Dcaf8 | S123, S124 | 2 | 1.5/−/− | Unknown |
| Dpysl3 | S101 | 1 | −/1.7/− | Unknown |
| Eif4b | [T420 >S422] | 1 | −/3.1/− | Increases interaction with Eif3 (88) and Eif4b activity (89) |
| Flnc | S2234 | 1 | 3.3/2.3/1.7 | Unknown |
| Fnbp1l | S295 | 1 | −/2.5/− | Unknown |
| Frs2 | T326 | 1 | 1.8/−/− | Unknown |
| Gm15128 | S384, S388 | 2 | −/1.5/− | Unknown |
| Gsta3 | T68, Y74 | 2 | −/−/1.8 | Unknown |
| Hmgn1 | [T70 = T71 >S84 = S87] | 2 | −/1.6/− | Unknown |
| Irf2bp2 | S421 | 1 | 2.2/−/− | Unknown |
| Irs2 | S556 | 1 | −/1.5/− | Unknown |
| Itpr3 | S934 | −/1.7/− | Unknown | |
| Kif21a | S855 | 1 | −/2.1/− | Unknown |
| Larp7 | [T251 >S253] | 1 | −/1.6/− | Unknown |
| Lima1 | S230 | 1 | −/1.5/− | Unknown |
| Map1b | S614 | 1 | −/1.6/− | Unknown |
| Map1b | S1793 | 1 | −/−/1.6 | Unknown |
| Mapk1 | [T179 >T183 = Y185 = T188] | 1 | −/8.7/− | pT183 and pY185 required for activation (90). Function of pT179 and pT188 unknown |
| Mapk1 | T183, Y185 | 2 | 11/−/− | |
| Mapk1 | T183 [Y185 = T188 >T179] | 2 | 9.5/−/− | |
| Mapk1 | Y185 [T179 = T183] | 2 | 5.4/−/− | |
| Mapk1 | Y185 | 1 | 2.4/2.4/− | |
| Mapk3 | T199 [T203 = Y205] | 2 | 6.7/−/− | pT203 and pY185 required for activation (90). Function of pT199 and pT208 unknown. |
| Mapk3 | Y205 | 1 | 3.5/2.9/− | |
| Mapk3 | T208 [T203 >Y205 >T199] | 2 | 4.8/−/− | |
| Mapk3 | [T208 >Y205] | 1 | −/3.0/− | |
| Matr3 | S188 | 1 | 1.7/1.8/− | Unknown |
| Matr3 | S195 | 1 | −/2/− | Unknown |
| Med19 | S226 >S234 = S235 = S238 = S239 = S240 = S241 = S242 | 7 | −/9.6/− | Unknown |
| Mia3 | S1915 | 1 | −/−/1.7 | Unknown |
| Myh9 | [T1938 = S1942] ([T1939 = S1943]) | 1 | 1.4/−/− | Unknown |
| Ncbp1 | S22 | 1 | −/2.2/− | Unknown |
| Ncoa5 | S29, S34 | 2 | −/1.6/− | Unknown |
| Ndrg1 | T328 | 1 | −/1.8/− | Primes NDRG1 for phosphorylation by GSK3 (65). Required for suppressive effect on NFκΒ signaling (66) |
| Ndrg1 | S330 | 1 | −/1.5/− | |
| Nr3c1 | T159 (T168) | 1 | −/1.8/− | Unknown |
| Oxr1 | S113 (S194) | 1 | 2.5/2.4/− | Unknown |
| Oxr1 | S114 (S195) | 1 | −/2.1/− | Unknown |
| Parva | T16, S19 | 2 | −/1.5/− | Unknown |
| Pdcd4 | S76 | 1 | −/3.5/− | Mediates Pdcd4 interaction with βTRCP (91) |
| Pdcd4 | S457 | 1 | −/3.8/− | Promotes in nuclear translocation of Pdcd4 (92) |
| Pgrmc1 | Y180 | 1 | 1.4/−/− | Unknown |
| Phf3 | [S658 >S660] | 1 | −/3.5/− | Unknown |
| Phldb2 | T468 | 1 | −/1.8/− | Unknown |
| Pi4k2a | [S460 >S462] | 1 | −/−/2.3 | Unknown |
| Pkn2 | [S581 = S582] | 1 | −/6.7/− | Unknown |
| Pkn2 | T957 | 1 | −/−/1.6 | Unknown |
| Plec1 | [T19 >S21] | 1 | −/−/1.6 | Unknown |
| Plec1 | [S4391 >S4392 >S4393] [(S4243 >S4244 >S4245)] | 1 | −/2.1/− | Unknown |
| Plec1 | S4393 (S4245) | 1 | 1.6/2.1/− | Unknown |
| Ppfibp1 | S957, S961 (S992, S996) | 2 | −/1.5/− | Unknown |
| Ppp1r12a | S507 | 1 | 6.9/8.6/− | Unknown |
| Ppp1r12a | S509 | 1 | 4.0/−/− | Unknown |
| Ppp1r12a | S861 | 1 | 1.5/−/− | Unknown |
| Ppp1r12a | [S870 >S877] | 1 | −/1.7/− | Unknown |
| Ptprn2 | S89 | 1 | −/104.9/− | Unknown |
| Rai14 | S914 | 1 | 1.5/−/− | Unknown |
| Ranbp3 | S58 | 1 | −/2.5/− | Important for nuclear export function (93) |
| Raver1 | S576 | 1 | −/1.9/− | Unknown |
| Rbm17 | S155 | 1 | 1.7/−/− | Unknown |
| Rbmx | S165 | 1 | −/1.5/− | Unknown |
| Rgs2 | S19 | 1 | −/12.2/− | Unknown |
| Rnf220 | T474 | 1 | −/−/5.6 | Unknown |
| Rptor | S863 | 1 | 1.5/2.0/1.9 | Required for hierarchical raptor phosphorylation. Plays a critical role in mTOR activity (57, 94) |
| Sdpr | S359 | 1 | 1.6/−/− | Unknown |
| Senp7 | S12 | 1 | −/2.7/− | Unknown |
| Sep9 | [S41 = T42] | 1 | 1.4/−/− | Unknown |
| Sgta | [S303 >T305] | 1 | −/2.0/− | Unknown |
| Shc1 | Y423 | 1 | −/3.7/− | Binds grb2 (61) |
| Sipa1l2 | S1461 | 1 | −/−/1.6 | Unknown |
| Slc9a1 (NHEI) | S707 | 1 | −/7.3/3.3 | Binds 14-3-3 and is required for Serum-induced activation of Nhe1 (95) |
| Srrm1 | [S461 >S463] | 1 | −/2.8/− | Unknown |
| Srrm2 | S2224 | 1 | −/2.9/− | Unknown |
| Stat5a | Y694 | 1 | −/+GH only/+GH only | Required for Stat5 activation (96) |
| Stat5b | Y699 | 1 | −/+GH only/+GH only | Required for Stat5 activation (96) |
| Stk10 | T950 | 1 | −/2/2.3 | Unknown |
| Stmn1 | S25 | 1 | −/7.6/− | Unknown |
| Tbc1d15 | S201 | 1 | −/−/1.6 | Unknown |
| Tnks1bp1 | S1022 | 1 | −/2.5/− | Unknown |
| Tnks1bp1 | S1375 | 1 | −/−/1.5 | Unknown |
| Tns3 | S769 | 1 | 2.0/−/− | Unknown |
| Top2b | S1387 (S1537) | 1 | 1.5/1.9/− | Unknown |
| Tpr | S2149 | 1 | −/6.3/− | Unknown |
| Trim28 | S471 | 1 | −/−/2.3 | Unknown |
| Trim28 | S473 | 1 | −/1.5/2.4 | Regulates Trim28 interaction with HP1β (97) |
| Trim47 | S591 (S592) | 1 | −/−/2.0 | Unknown |
| Trio | S2399, S2403 (S22575, 2579) | 2 | −/5.0/− | Unknown |
| Tsc2 | [S939 >T940 >S941] [(S937 >T938 >S939)] | 1 | −/1.9/− | Promotes interaction with 14-3-3 and relieves inhibition of mTOR signaling (98) |
| Twist1 | S68 | 1 | 1.6/−/− | Unknown |
| Twist2 | S55 | 1 | 1.6/−/− | Unknown |
| Usp10 | S223 | 1 | −/3.8/− | Unknown |
| Wdr48 | S586 | 1 | −/−/5.0 | Unknown |
| Zc3hc1 | S394 | 1 | −/−/1.8 | Unknown |
| Zc3hc1 | S406 | 1 | −/2.6/− | Unknown |
| Zyx | S336 | 1 | −/1.8/− | Unknown |
2
Numbering of residues is according to PhosphositePlus (http://www.phosphosite.org). Numbering in parentheses is according to MaxQuant. Numbering in brackets refers to unlocalized sites (localization probability <0.75 and >0.15).
3
5a and 5b refer to the first and second 5 min trials, respectively.