Table 3.
GH-stimulated phosphosites identified by phosphoproteomics that are predicted4 to be phosphorylated by Akt/PKA
| Gene | Protein | Phosphosite5 | Ratio (+GH/−GH) (5a/5b/15 min)6 |
|---|---|---|---|
| Acly | ATP citrate lyase (ACLY) | Ser455 | 1.5/−/− |
| Akt1s1 | Proline-rich AKT1 substrate 1 (PRAS40) | Thr247 | 3.8/−/− |
| Bcl9l | B-cell CLL/lymphoma 9-like protein | Ser118 | −/2.4/− |
| Dact3 | Dapper homolog 3 | Ser409 | 2.2/−/− |
| Eif4b | Eukaryotic translation initiation factor 4B (eIF4B) | Thr420 | −/3.1/− |
| Flnc | Filamin-C | Ser2234 | 3.3/2.3/1.7 |
| Ndrg1 | N-myc downstream-regulated gene 1 protein (NDRG1) | Thr328 | −/1.8/− |
| Ndrg1 | N-myc downstream-regulated gene 1 protein (NDRG1) | Thr330 | −/1.5/− |
| Zc3hc1 | Nuclear-interacting partner of anaplastic lymphoma kinase (Nipa) | Ser406 | −/2.6/− |
| Pdcd4 | Programmed cell death protein 4 | Ser457 | −/3.8/− |
| Plec1 | Plectin-1 | Ser4391 (Ser4243) | −/2.1/− |
| Plec1 | Plectin-1 | Ser4393 (Ser4245) | 1.6/2.1/− |
| Ranbp3 | Ran-binding protein 3 | Ser58 | −/2.5/− |
| Slc9a1 | Sodium/hydrogen exchanger 1 (NHE1) | Ser707 | −/7.3/3.3 |
| Tns3 | Tensin-3 | Ser769 | 2.0/−/− |
4
GH-dependent phosphopeptides identified during the SILAC-based phosphoproteomics screens were analyzed by MaxQuant to predict phosphosites with best-fit motif consistent with phosphorylation by Akt.
5
Numbering is according to PhosphositePlus (http://www.phosphosite.org). Numbering in parentheses is according to MaxQuant.
6
5a and 5b refer to the first and second 5 min trials, respectively.