Figure 1.

Dissecting the glycosylation machinery. Glycosylation is a complex process that involves a large number of molecules and organelles. The glycosylation machinery can be defined as the set of enzymes, chaperones, transporters, sugar donors, and accessory molecules necessary for the modification of proteins or lipids with carbohydrates. Since many of these molecules are subjected to regulation, glycosylation is a highly dynamic process and it is, therefore, interesting to address not only the array of glycans present on the cell surface or the secretome, but also the activity and the expression levels of the molecules involved in glycan biosynthesis.