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Ethanol increases Vamp2 gene expression. (A) Vamp2 mRNA levels increase after 1 h treatment with different concentrations of ethanol, as measured by qPCR. The half-maximal activation of Vamp2 was calculated as 40 ± 6 mM [n ≥ 6; F(9, 72) = 20.45; p < 0.0001]. (B) Vamp2 mRNA levels increase after 60 mM ethanol exposure over time [n ≥ 6; F(10, 195) = 39.58; p < 0.0001; *P < 0.05, ***P < 0.001].
