Figure 3.

OXTR ligand binding in the neocortex is prominent in layer II/III. (A) OXTR is abundant in neocortical layers II/III at P14. (B) Nissl counterstain of section in (A). (C) OXTR is pseudo-colored red in a composite image of panels (A) and (B), which indicates that OXTR is prominent in layers II/III but not in layer IV or VI. OXTR is present in upper layer V. There is significantly reduced neocortical OXTR in P60 mice (D) which are only slightly above tissue background compared to OXTR KO P60 neocortex (E). (F) Quantification of OXTR binding demonstrates the transient ligand binding of OXTR in upper layers across post-natal development.