Figure 1.

Derivation of Immature Intestinal Progenitors from Human Fetal and Pluripotent Cells

(A) Whole mount of human gestational week 10 small intestine.

(B) Higher magnification of villi (arrow) and intervillus regions (arrowhead) in (A).

(C–E) Immunohistochemistry analysis for Ki67 (C), PAS staining (D), and Lysozyme (E) in week 10 human small intestine.

(F and G) Spheroid cultures from week 10 human small intestinal epithelium, grown with (G) and without (F) prostaglandin E2 (PGE2) (2.5 μM).

(H and I) Intestinal tissue derived from directed differentiation of human induced pluripotent stem cells (hiPSCs), cultured with (I) and without (H) PGE2.

(J) Relative expression levels of intestinal lineage markers in material from undifferentiated human induced pluripotent stem cells (hiPSC), iPSC-derived intestine (Int. diff.), human primary fetal enterospheres (hFEnS), human adult organoids (hOrgs), primary fetal human small intestine (FhSI), and primary adult human small intestine (AhSI). Red and green colors reflect increased and decreased deviation from the mean, respectively.

(K) Detection of VILLIN (green) and CHGA (red) in hiPSC-FEnS.

The scale bars represent 2 mm in (A) and 100 μm in (C)–(E) and (K). See also Figure S1 and Table S1.