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Rate constants derived from kinetic analysis of the assembly lines shown in Figure 1.
| Assembly Line | Component Varied | (V/[E]0)max(min−1) | K50(μM) |
|---|---|---|---|
| LDD-M1-M2+TE | M1 | 21 ± 0.9 | 7.3 ± 0.7 |
| M2+TE | 15 ± 0.7 | 6.3 ± 0.9 | |
| LDD-M1-M2-M3+TE | M2 | 2.1 ± 0.1 | 2.6 ± 0.2 |
| M3+TE | 2.5 ± 0.1 | 3.2 ± 0.4 | |
| LDD-M1-M2-DEBS2-DEBS3 | DEBS2 | 1.1 ± 0.1 | 4.0 ± 1.1 |
| DEBS3 | 0.8 ± 0.1 | 2.9 ± 0.5 | |
