Figure 4.

E/R expression induces DNA damage in wt and E/R^tg cells. (A) wt and E/R^tg cell lines were stained for γH2AX and analyzed by flow cytometry. One representative histogram is shown. (B and C) Comet assay of MACS-enriched CD19⁺ B cells derived from BM of wt and E/R^tg mice. Two mice of each genotype were pooled before MACS. (B) Representative pictures of comets of each genotype. (C) Statistical analysis (unpaired t test) of comet assay of wt and E/R^tg mice (n = 6 each) is depicted. Fifty pictures per sample were taken for statistical analysis of percentage of tail DNA. Average ± SD of each sample is shown.