Figure 4.
Nucleic acid extraction and purification from cell lysate. RNA from MCF-7 cell lysate was purified using capillary and conventional washing methods. Amplification of ribosomal protein P0 demonstrated similar threshold cycle values between the two purification methods. A linear response was observed across the different cell concentration with both techniques as shown by the R2 values. Data and error bars represent the mean and the standard deviation of the triplicate experiment.