Fig. 2.

Western blot analysis of (A) VEGF-A and (B) Angpt-2 after variable cryopreservation methods and quantitative analysis of each group. β-Actin was used as internal standard. The data represents mean±standard error of three independent experiments. DMSO, dimethyl sulfoxide; EFS, 40% EG, 18% ficoll, 0.5 mol/L sucrose, and 20% FBS in modified Dulbecco's phosphate buffered saline; Angpt-2, angiopoietin-2; VEGF-A, vascular endothelial growth factor-A. ^a)P<0.05 vs. cryopreservation groups (vitrification using DMSO, EFS, and slow freezing); ^b)P<0.05 vs. other cryopreservation groups (vitrification using DMSO and slow freezing).