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. 2013 Oct 3;13(10):13425–13438. doi: 10.3390/s131013425

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 2. — Electrophoretic Mobility Supershift Assay of Vap7-NH₂ + VEGF₁₆₅ + VEa5-FAM (a) and of VEa5-NH₂ + VEGF₁₆₅ + Vap7-FAM (b). In Panel (a) the Vap7-NH₂-VEGF₁₆₅-VEa5-FAM interaction was analyzed, while in Panel (b) the VEa5-NH₂-VEGF₁₆₅-Vap7-FAM interaction was analyzed. Each aptamer was incubated separately or simultaneously with the protein under the described conditions. The respective aptamer and VEGF₁₆₅ concentrations are indicated in the figure. Binding reactions were applied on a 12% non-denaturing PAA gel containing glycine buffer and 10 mM KCl. The mobility of free and complexed aptamers, detected by the FAM fluorescence, was analyzed using the Geliance 600 Imaging System.