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Single site substitutional analysis based on the MycP1 substrate Abz-AVKAASLG using ProteaseSpot™ system. Bars represent the fluorescent signal from peptide fragments released from the cellulose membrane over 2 hours. Fluorescence is directly proportional to the activity of MycP1mth against each peptide. The relative activity of MycP1mth against peptides was normalized such that activity versus EspBmtu peptide sequence (Abz-AVKAASLG) = 1.0.
