Skip to main content
. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Mol Microbiol. 2013 Oct 7;90(4):10.1111/mmi.12398. doi: 10.1111/mmi.12398

Figure 3.

Figure 3

The C-terminal region of PopZ is necessary for sub-cellular localization and oligomer formation.

A. Schematic of PopZ truncations.

B. Images of strains expressing full length mVenus-PopZ (AP323) (WT), mVenus-PopZΔ134–177 (AP303), mVenus-PopZΔ160–177 (AP322), and mVenus-PopZΔ172–177 (AP305) in a ΔpopZ background. Venus fluorescence (green) overlays the phase contrast image (grayscale).

C. Percent of polar localized PopZ signal in B.

D. Average cell length of strains expressing WT PopZ (GB699), no PopZ (GB255), PopZΔ134–177 (GB885), PopZΔ160–177 (GB886), and PopZΔ172–177 (GB888) in a ΔpopZ background.

E. Localization of SpmX-mCherry in PopZ variant backgrounds. Strains in B are modified to express SpmX-mCherry from the chromosomal spmX promoter. Images from AP253, AP236, AP280, AP300, and AP282 are presented. SpmX-mCherry fluorescence (red) overlays the phase contrast image (grayscale).

F. Percent of polar localized SpmX-mCherry signal in PopZ variant strains, shown in E.

G. Electrophoretic migration of PopZ truncations. Whole cell lysates of strains in panel D were resolved on native and denaturing gels, then probed with anti-PopZ sera by immunoblotting.

In images, Bar = 1µm. In graphs, error bars represent SEM from 2 separate experiments of 30–60 cells each.