Table 1.
Size and shape of PopZ complexes determined by SEC-LS
| sample | Concentration at peak elution (mg/ml) |
Calculated Molecular weight (kDa)a |
calculated Rg (nm)b |
calculated Rh (average, nm)c |
|---|---|---|---|---|
| 6His-WT | 0.007 | 122 (+/− 5%) | 8.6 | |
| 6His-WT | 0.059 | 163 (+/− 5%) | 12.0 | |
| 6His-WT | 0.230 | 202 (+/− 5%) | 18.0 | 11.5 (+/− 0.3) |
| 6His-Δ172–177 | 0.008 | 128 (+/− 5%) | 9.3 | |
| 6His-Δ172–177 | 0.085 | 121 (+/− 5%) | 9.7 | |
| 6His-Δ172–177 | 0.253 | 127 (+/− 5%) | 9.3 | 9.1 (+/− 0.3) |
| 6His-P146A | 0.014 | 64 (+/− 5%) | 10.0 | |
| 6His-P146A | 0.131 | 66 (+/− 5%) | 8.0 | |
| 6His-P146A | 0.22 | 71 (+/− 5%) | 13.0 | 6.8 (+/− 0.3) |
| apoferritin | 475 (+/− 5%) | 6.4 | 6.9 (+/− 0.3) |
a
Average value determined from all incident light angles.
b
Determined from first order Zimm fitting, excluding low angle (<45° ) signal coming from large particles.
c
Due the limited sensitivity of dynamic light scattering, this value could only be determined in fractions with high protein concentrations.