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IL12 and IL10 production by 3-day and 7-day GM-CSF/IL4 cultured monocytes in response to Poly(I:C)/IFN-γ. DCs in each case were matured by 18 h exposure to the maturation cocktail. IL12 (A and B) and IL10 (C and D) were measured in culture medium collected immediately after maturation (A and C) or after harvest and 18 h restimulation with CD40L (B and D). Statistically significant reductions in IL12 production after restimulation were noted.
