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. Author manuscript; available in PMC: 2013 Dec 11.
Published in final edited form as: Science. 2011 Jun 17;332(6036):10.1126/science.1196766. doi: 10.1126/science.1196766

Fig. 2.

Fig. 2

PERs, PSF, and the SIN3-HDAC complex. (A) Co-immunoprecipitation of endogenous PER2 and PSF with SIN3A. Nuclear extracts (CT18) from liver or lung (input) and immunoprecipitates (IP, antibodies at top) from the extracts were probed with antibodies at right. U2AF65 and LaminA/C, negative controls; IgG-LC (light chain), positive control. (B) Synchronous circadian cycle of PER2, CRY2, PSF, SIN3A, and HDAC1 at Per1 proximal E-box site. ChIP assays from lungs sampled across a circadian cycle (bottom) performed with antibodies at the top left of each panel. ChIP values are relative to the signal at an arbitrary internal control genomic region (SOM methods). (C) Top, diagram of mouse Per1 gene showing positions of proximal E-box and control sites C1, C2, and C3. Bottom, ChIP assays from lungs harvested at CT10-14, performed with antibodies against the indicated proteins. ChIP data show mean +/− SEM of triplicate experiments; representative of 2-4 experiments.