Skip to main content
. Author manuscript; available in PMC: 2013 Dec 11.
Published in final edited form as: Science. 2011 Jun 17;332(6036):10.1126/science.1196766. doi: 10.1126/science.1196766

Fig. 4.

Fig. 4

Depletion of SIN3A increases Per1 transcription and shortens circadian period length. (A) Quantitative RT-PCR assays, as in Fig. 1B, showing steady-state abundance of indicated pre-mRNAs in fibroblasts after introduction of point-mutant control SIN3A shRNA (white) or after depletion of SIN3A by an effective SIN3A shRNA (black). Shown are means +/− of triplicate experiment; representative of 3 experiments. (B-D) Short circadian period length caused by depletion of endogenous SIN3A from fibroblasts. (B) Western blot showing the effect of mutated control (Mut) siRNA or SIN3A siRNA on steady-state level of endogenous SIN3A. ACTIN, loading control. (C) Circadian oscillations of bioluminescence in synchronized circadian reporter fibroblasts after delivery of control Mut SIN3A siRNA (yellow) or SIN3A siRNA (blue). Traces from three independent cultures are shown for each condition. (D) Circadian period of fibroblasts with control Mut SIN3A siRNA or SIN3A siRNA (mean +/− SEM; N = 3; t-test, two-tailed).