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. 2013 Jul 15;20(6):525–535. doi: 10.1093/dnares/dst028

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© The Author 2013. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — (A) Levels of G6PD and 6PGD in the GT and GOX50 strains under nitrogen-limited conditions, assayed by immunoblotting (total protein 12 μg). Bands show the relative expression of the two proteins under nitrogen-replete conditions (+N) and after 1 and 3 days of nitrogen depletion (−N). Data represent mean ± SD results from six independent experiments. Protein levels were calibrated relative to that of the GT strain under nitrogen-replete conditions (set at 100%). Statistically significant differences between GT and GOX50 are marked by asterisks (Student's t-test; *P < 0.05, **P < 0.005). (B) Enzyme activities of G6PD and 6PGD under nitrogen-replete and -depleted conditions. Data represent mean ± SD of values from three independent experiments.