Figure 3.

Methylated and hydroxymethylated mtDNA immunoprecipitation. AluI-digested DNA extracted from blood and cultured cells of human and mouse were immunoprecipitated with anti-5mC (A) and anti-5hmC (B). Samples were amplified with primers specific for DNA fragments detected as unmethylated and methylated by bisulphite sequencing. The nucleotide position of these fragments is indicated. Fold enrichment was calculated as the ratio of amplification efficiency of the immunoprecipitated sample over that of non-immune IgG and normalized to the nanograms of immunoprecipitated DNA amount. Data represent the means of six triplicate experiments with standard errors of the mean.