Figure 4. Application of the acid phosphatase (APH) assay to determine drug effects in MIAPaCa-2 and PANC-1 spheroid cultures. APH activity/cell viability in MIAPaCa-2 and PANC-1 monolayer and spheroid cultures after 72 h of treatment with gemcitabine and 5-fluorouracil (5-FU). Data are reported as means of ≥3 individual experiments each with eight spheroids treated and measured per condition. IC50 values were calculated to investigate the difference of drug efficacy in 2-D vs 3-D culture and in these two pancreatic cancer cell lines.
