Table 2.
Name | RE Site Additiona | Sequenceb |
---|---|---|
CHSFP | None | 5′-AGGCAAGACATGGTGGT-3′ |
CHSRP | None | 5′-GGAACATCCTTGAGGAG-3′ |
CHS1FP | None | 5′-GCAAAAACTTAAAGTTGGAATAAAATTTGGC-3′ |
CHS1RP | None | 5′-CATCCTAGCTGGTTAAGAAAAGAATGGA-3′ |
DnaJ FP | None | 5′-AAAACGACAGCTAATCACGC-3′ |
DnaJ RP | None | 5′-TCTGGAAGGATCAGACAGGG-3′ |
1 | None | 5′-CACAATACGTTTTTTCAAACCGG-3′ |
2 | None | 5′-TTCCCCCTGCCCTGCAAATGCTTC-3′ |
3 | EcoRI | 5′-GCCGAATTCGAAACACAATACGTTTTTTCAAACCGGCCAGCC-3′ |
4 | EcoRI | 5′-GCCGAATTCCCTGCAAATGCTTCTTTTTGTATACCAG-3′ |
5 | None | 5′-TCCAGGGTGATCCTATGGAAGGACTGACCC-3′ |
6 | EcoRI | 5′-GCCGAATTCCATTATGCATTGCAATAAGATGGGGTCAGG-3′ |
7 | None | 5′-ATGCGTTCTTATGGCTTAACCG-3′ |
Restriction enzyme is abbreviated as RE.
Extra restriction enzyme sites added are underlined.