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. 2013 Dec 1;63(4):384–392. doi: 10.1270/jsbbs.63.384

Table 2.

Sequences of primers used in PCR analyses

Name RE Site Additiona Sequenceb
CHSFP None 5′-AGGCAAGACATGGTGGT-3′
CHSRP None 5′-GGAACATCCTTGAGGAG-3′
CHS1FP None 5′-GCAAAAACTTAAAGTTGGAATAAAATTTGGC-3′
CHS1RP None 5′-CATCCTAGCTGGTTAAGAAAAGAATGGA-3′
DnaJ FP None 5′-AAAACGACAGCTAATCACGC-3′
DnaJ RP None 5′-TCTGGAAGGATCAGACAGGG-3′
1 None 5′-CACAATACGTTTTTTCAAACCGG-3′
2 None 5′-TTCCCCCTGCCCTGCAAATGCTTC-3′
3 EcoRI 5′-GCCGAATTCGAAACACAATACGTTTTTTCAAACCGGCCAGCC-3′
4 EcoRI 5′-GCCGAATTCCCTGCAAATGCTTCTTTTTGTATACCAG-3′
5 None 5′-TCCAGGGTGATCCTATGGAAGGACTGACCC-3′
6 EcoRI 5′-GCCGAATTCCATTATGCATTGCAATAAGATGGGGTCAGG-3′
7 None 5′-ATGCGTTCTTATGGCTTAACCG-3′
a

Restriction enzyme is abbreviated as RE.

b

Extra restriction enzyme sites added are underlined.