Figure 1. HOCl reversibly inhibits TNF-α–stimulated NF-κB–dependent gene expression in primary human keratinocytes.

(A) Relative mRNA levels of CCL2 and SOD2 in TNF-α–stimulated human keratinocytes with (dotted line) and without (solid line) HOCl pretreatment. (B) Cell viability assessed by trypan blue after HOCl treatment. (C) Relative mRNA levels of CCL2 and SOD2 in human keratinocytes pretreated with H₂O or HOCl; 24 hours later, cells were stimulated with TNF-α for 2 hours. Control represents untreated keratinocytes. Data are presented as the average ± SEM. Conditions with and without HOCl treatment are denoted by black and gray bars, respectively.