Figure 6. Inhibition of muscarinic receptor–mediated TRPC5 activity prevents podocyte damage.

(A) The muscarinic receptor agonist Cch induces a significant increase in Ca²⁺ transients in podocytes located on acutely isolated glomeruli from WT mice but not from Trpc5-KO mice. (B) Quantification of averaged peak amplitude at Δt = 1 min (n = 11 glomeruli per group). (C) Peak amplitude of Cch-induced Ca²⁺ transients in podocytes on acutely isolated glomeruli was attenuated by 3 μM ML204. (D) Quantification of averaged peak amplitude at Δt = 1 min (n = 12 glomeruli [Cch], 10 glomeruli [Cch+ML204]). (E) Loss of stress fibers induced by 100 μM Cch was blocked by ML204. (F) Significant rescue of stress fiber formation by ML204 (n = 90 images/condition). Scale bar: 50 μm (C). Original magnification, ×400 (E). **P < 0.01, ***P < 0.001, Student’s t test (B and D); ****P < 0.00001, ANOVA (F).