Figure 3. DREAMM imaging reveals that neuronal inhibition of Pdyn neurons in the PAC increases metabolic activity in the ExA.

(A) Axial and right sagittal views showing that activation of G_i-mediated signaling in Pdyn neurons of the PAC with CNO leads to a profound induction of neuronal activity primarily in the right ExA as compared with vehicle administration in the same animal (red, relative increase; blue, relative decrease). LH, lateral hypothalamus; NAcSh, NAc shell; SI, substantia inominata; VMH, ventral medial hypothalamus; IPAC, interstitial nucleus of posterior limb of anterior commissure; SC/MC, sensory cortex/motor cortex; ON, olfactory nuclei; CeA, central amygdala; MVePC,: medival vestibular nucleus, parvicellular part; VP, ventral pallidum; LEnt, lateral entorhinal cortex; BLA, basolateral amygdala; MPA, medial preoptic area. (B) Schematic diagram of the ExA circuit and its substructures.