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. 2013 Nov 1;123(12):5319–5333. doi: 10.1172/JCI71171

Figure 8. Heterozygous deletion of Becn1 decreases autophagy and attenuates heart failure in CIRS12KO mice.

Figure 8

(A) Representative H&E stains from WT and CIRS12KO hearts (6 weeks of age) expressing Becn1+/+ or Becn1+/– alleles. Scale bar: 3 mm. (B) Survival curve of WT, Becn1+/–, CIRS12KO, and CIRS12KO×Becn1+/– mice. Heterozygous deletion of Becn1 increased the average lifespan of CIRS12KO mice from 7.4 to 16.8 weeks (P < 0.0001, log-rank test). (C) Autophagy flux after chloroquine injection at 2 weeks of age, as measured by LC3 immunoblotting. (D) Heart weight/tibia length and (E) wet lung weight/tibia length ratios. (F and G) Representative H&E and trichrome staining (F) and stereological analysis (G; n = 6–7) in hearts from 6-week-old mice. Scale bar: 20 μm. (H) Time course for LVDs and FS (n = 5–10). (I) Invasive measurement of LV pressures in WT and CIRS12KO hearts expressing Becn1+/+ or Becn1+/– alleles at 6 weeks of age, as assessed by catheterization. *P < 0.05 vs. same Becn1 genotype, †P < 0.05 vs. same Irs genotype, ‡P < 0.05 vs. same genotype no chloroquine.