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(A–H) Western blot analysis of VEGF-induced MAPK p44/42 Tyr202/Tyr204, Akt Ser473, and VEGFR-2 Tyr1175 phosphorylation following siRNA-mediated PTP1B (A) or TCPTP (E) downregulation in Esdn–/– (KO) and WT MLECs. Quantitative analysis of the ratio of phosphorylated to total MAPK and VEGFR-2 from three independent experiments is shown in B–D and F–H.
