Figure 6. Overexpression of Atg7 significantly reduced misfolded protein accumulation in CryAB^R120G hearts.

(A) Immunofluorescence staining for CRYAB (green) with troponin I counterstaining (red) showed decreased aggregate levels in CryAB^R120G×Atg7×tTA cardiomyocytes. (B) Quantification of CryAB aggregates showed decreased aggregate accumulation in CryAB^R120G×Atg7×tTA mice. (C) Representative Western blot showing aggregated CRYAB protein levels present in the insoluble fractions. (D) Densitometry analysis showed decreased aggregate in the insoluble protein fraction in CryAB^R120G×Atg7×tTA mice (n = 6 per group). P values were determined by Tukey’s post-hoc test. Scale bars: 10 μm.