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. 2013 Nov 25;123(12):5269–5283. doi: 10.1172/JCI63428

Figure 2. Assessment of LIF-stimulated cell proliferation in NPC cells.

Figure 2

(A) Real-time cell proliferation assays of TW06 cells treated with LIF, sLIFR, or PBS (control). sLIFR (1 μg/ml) was added to the growth medium of TW06 cells 2 hours prior to LIF treatment. Black arrows indicate the time at which LIF and sLIFR were added. Values are presented as means and SD of triplicate experiments. (B) Analysis of EdU incorporation in CNE1 cells treated with PBS, LIF, sLIFR, or sLIFR plus LIF for 24 hours, followed by EdU labeling for 3 hours. EdU+ cells were quantified using ImageJ software. Overall P < 0.001, 1-way ANOVA; *P < 0.05; **P < 0.01; ***P < 0.001, Bonferroni’s multiple comparison test. Scale bar: 50 μm. (C) Bioluminescent images of NPC tumor xenografts treated with PBS, LIF, or sLIFR. Images were first detected on the seventh day after implantation of TW06_Luc2 cells. Drug administration was started on the eighth day after implantation and continued twice per week for 6 weeks. (D) Quantification of the total photon fluxes from the tumors shown in C. *P < 0.05; **P < 0.01, paired t test, compared with PBS-treated group. Values are presented as means and SEM (n = 5 mice per group).