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. 2013 Nov 25;123(12):5269–5283. doi: 10.1172/JCI63428

Figure 3. LIF activates the p70S6K-signaling pathway.

Figure 3

(A) Protein expression of LIFR, gp130, and LIF in 4 NPC-derived cell lines: C666-1, CNE1, TW01, and TW06. Right panel showed levels of secreted LIF in the 4 NPC cell lines. Cell-free culture supernatants were collected at 36 hours after plating, and LIF was measured by bead-based assays (Bio-Rad). (B–E) Western blotting analyses of signaling protein and its phosphorylated form using site-specific antibodies. GAPDH was used as a loading control. (B) LIF dose dependently activates p70S6K and its downstream targets in CNE1 cells. Protein lysates were harvested 10 minutes after LIF treatment. (C) Time-course analysis of p70S6K signaling molecules in LIF-treated CNE cells. (D and E) Pretreatment with sLIFR (1 μg/ml) 2 hours prior to LIF treatment prevents LIF-induced activation of p70S6K signaling in both TW06 cells (D) and CNE1 cells (E).