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. 2013 Nov 25;123(12):5269–5283. doi: 10.1172/JCI63428

Figure 8. EBV-encoded LMP1 activates LIF expression.

Figure 8

(A) Assessment of LMP1-induced upregulation of LIF mRNA in TW06 cells. Cells were transfected with various doses of LMP1-expressing or control vectors. (B) Quantification of secreted LIF in cell-free culture supernatants collected at 24 hours after transfection in TW06 cells. (C) Western blotting analysis of LIF and p-IκB in TW06 cells transfected with increasing doses of LMP1. Protein lysates were harvested at 24 hours after transfection. GAPDH was used as the loading control. (D–F) Mutations or deletion in the CTAR domains of LMP1 affect expression of LIF. Total RNA, culture supernatants, and protein lysates were harvested at 24 hours after transfection in TW06 cells. Relative LIF mRNA expression (D), secreted LIF (E), and LIF expression in protein lysates (F) were shown. mRNA expression was normalized to that of COL4A6 (Supplemental Table 3), which showed unchanged expression levels across NPC microarray experiments (GSE14262). GAPDH was used as the loading control of proteins. *P < 0.05; **P < 0.01, paired t test.