Table 1.

Experimental Melting Temperatures of RNA Duplexes in Various Sodium Ion Concentrations^a

		Tmb (°C)
RNA sequence (5' to 3')d	fGCe	71 mM	121 mM	221 mM	621 mM	1.021 Mc
CGCGCG	1.00	51.3	53.7	55.2	57.8	57.8
CGGCCG	1.00	55.2	57.4	59.9	61.9	63.2
GCCGGC	1.00	60.1	62.6	65.0	67.6	67.4
GCGCGC	1.00	55.3	57.8	59.9	62.3	62.5
ACCGGU	0.67	43.7	47.2	50.1	52.0	53.9
AGCGCU	0.67	41.3	45.7	47.9	51.4	52.0
CACGUG	0.67	33.6	36.2	38.5	41.0	42.8
CAGCUG	0.67	35.3	37.2	39.7	42.1	43.1
CCAUGG	0.67	35.7	37.0	40.4	43.5	46.4
CCUAGG	0.67	39.7	42.4	44.7	47.4	50.0
CUGCAG	0.67	37.1	39.9	41.9	44.8	45.3
GACGUC	0.67	38.5	41.2	43.1	46.0	46.2
GAGCUC	0.67	40.2	42.7	45.0	47.7	48.7
GCAUGC	0.67	37.8	40.2	42.6	45.8	45.7
AACUAGUU	0.25	34.0	37.0	40.0	43.8	45.7
ACUAUAGU	0.25	32.9	36.2	38.8	42.7	44.0
ACUUAAGU	0.25	29.8	32.4	35.8	39.3	40.3
AGAUAUCU	0.25	31.0	33.9	37.7	41.7	41.4

^aT_m values are from the 1/T_m vs ln C_T plots.

^bCalculated for 0.1 mM oligomer concentration.

^cAll 1.021 M data, except for GCCGGC and GCGCGC, are from Xia et al. (9).

^dAll oligomers are self-complementary and are forming duplexes in solution.

^ff_GC is the fraction of G-C base pairs.