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. 2013 Dec 11;8(12):e81349. doi: 10.1371/journal.pone.0081349

Figure 2. E. ulmoides Oliver extract reduces hepatic lipid accumulation and levels of secreted apolipoprotein B.

Figure 2

(A) Cells were treated with 300 μM palmitate in the absence or presence of 100 μg/mL EUE for 12 hours. Fat accumulation was determined by Oil Red O staining. Images of cells were obtained at 200X original magnification and used for quantitative analysis of cellular lipid deposition (right). * p<0.05, significantly different from cells treated with palmitate alone. (B) Cells were treated with 300 μM palmitate in the presence or absence of 100 μg/mL EUE for 0, 3, 6, 9, 12, 18, or 24 hours. Cell lysates and media samples were subjected to immunoblot analysis with anti-ApoA1 or anti-ApoB. CBB staining was performed as an equal loading control. (C) Cells were treated with 300 μM palmitate in the absence or presence of 100 μg/mL EUE for 0, 6, 12, 24, or 48 hours. Triglyceride and cholesterol levels were measured for both cell lysates and media alone. * p<0.05, significantly different from cells treated with palmitate alone at the corresponding time point. Pal, palmitate; EUE, E. ulmoides Oliver extracts; CBB, Coomassie brilliant blue.