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. Author manuscript; available in PMC: 2013 Dec 11.
Published in final edited form as: J Can Res Updates. 2012 Jan 1;1(1):10.6000/1929-2279.2012.01.01.09. doi: 10.6000/1929-2279.2012.01.01.09

Fig. 1. PAX3-FKHR regulates the expression of PTN.

Fig. 1

(A) The mRNA levels of PAX3-FKHR (PF) or PTN in Rh30 (left panel) or Rh41 cells (right panel) transiently transfected with NT siRNA (Rh30_NT siRNA and Rh41_NT siRNA) were set as 1, and the mRNA levels of PF or PTN in cells transiently transfected with PF siRNA (Rh30_PF siRNA and Rh41_PF siRNA) or in RD were expressed as “mRNA fold change” by normalizing to the corresponding control. (B) RD, NIH3T3 (3T3), and KD1 cells were transiently transfected with either GFP empty vector or GFP-PAX3-FKHR (GFP-PF). The mRNA levels of PTN in 3T3_GFP, RD_GFP, or KD1_GFP were arbitrarily set as 1. (C) Protein levels of GFP-PF in cells (3T3, RD, or KD1) transiently transfected with either GFP or GFP-PF, detected by using an anti-GFP antibody. Actin was an equal loading control.