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. Author manuscript; available in PMC: 2013 Dec 11.
Published in final edited form as: J Can Res Updates. 2012 Jan 1;1(1):10.6000/1929-2279.2012.01.01.09. doi: 10.6000/1929-2279.2012.01.01.09

Fig. 3. PAX3-FKHR binds to the PTN promoter.

Fig. 3

(A) EMSA analysis of GFP-PAX3-FKHR binding to PTN promoter. All lanes contain labeled PTN probe in addition to other components as indicated. A 100-fold excess of unlabeled wild-type (PTN) or PD binding site mutant (PTN_m) was used to compete with the labeled PTN DNA probe for GFP-PAX3-FKHR binding. GFP-PF, nuclear extract prepared from HEK293T cells transfected with GFP-PAX3-FKHR; GFP, nuclear extract prepared from HEK293T cells transfected with GFP vector only. Arrow indicates the PAX-FKHR-DNA complexes. The amount of GFP-PF and GFP used for the EMSA is shown in the lower panel, using an anti-GFP antibody. (B) EMSA analysis of PAX3-FKHR binding to PTN promoter using in vitro translated PAX3-FKHR (PF) or vector control (pcDNA). The amount of PF used for the EMSA is shown in the lower panel by using anti-FKHR antibodies.