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. 2013 Dec 11;8(12):e82069. doi: 10.1371/journal.pone.0082069

Figure 2. Graphical representation of 44 isolates belonging to MLST-CC235 analyzed by PFGE, DL, MLST and MLVA.

Figure 2

A; Dendrogram generated by PFGE banding pattern, the clustering was done by UPGMA using the Dice coefficient with a tolerance position of 1%. 19 PFGE types were obtained with a cut-off of 80% of similarity. B; Dendrogram generated by the Pearson cluster analysis of rep-PCR results. PFGE-type, ST, MT and virtual gel are shown for each isolate. STs are depicted in colored boxes. A similarity index cut-off value of 95% was used by the DiversiLab® software to define genetic classification and to highlight the 9 DL-types. C; Minimum Spanning Tree illustrating the clonal complex MLST-CC235, the predominant ST235 is the primary founder surrounded by its SLVs (STs 976, 230, 989 and 227). Each color was assigned to each distinct ST. D; Minimum Spanning Tree of MLST-CC235 isolates typed by MLVA, Each circle represents an MT and 13 different colors were assigned to each distinct MT.