Figure 2. Cp-PLC rN-domain interacts with rC-domain.

(A) Co-precipitation of N and C-domains. 12% SDS-PAGE analysis of purified rC (lane 1), rN (lane 2) and co-precipitated eluate of rC and rN (lane 3). The procedure was described in the main text. (B) Far-dot ELISA analysis of the interaction between rN and rC-domains. Nitrocellulose membranes containing spotted rC and rN (Positive control - P) and blocked with 5% milk solution were probed with rN and detected with mouse anti-rN primary antibodies. No immuno-reactive dot is present in PBS control (N).