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. 2013 Dec 11;8(12):e82514. doi: 10.1371/journal.pone.0082514

Figure 6. Self-quorum quenching regulates flagellar genes expression.

Figure 6

(A) The relative abundance of fliF, flgE, flbT, and fliC mRNAs was determined by qRT-PCR on RNA isolated from bacteria harvested at the early exponential growth phase in 2YT supplemented or not with exogenous C12-HSL (5 µM). Deletion of aibP and addition of exogenous C12-HSL on B. melitensis both result in significant downregulation of the expression of the tested genes (P<0.001 in Student’s t test). ▵aibP PaibP (right panel) is the complemented strain. Results are representative of two independent experiments. Error bars represent standard deviation from biological triplicates. (B) (Left panel) Western Blot analysis of FliC and FlgE production performed on whole protein lysates of bacteria harvested at the early log phase of growth in 2YT. ▵aibP PaibP is the complemented strain. (Right panel) Bacteria were harvested at the early log phase of growth in 2YT in the absence (–) or in the presence of C12-HSL. The FliC and FlgE proteins were detected at their expected size (29 kDa and 41 kDa respectively). Detection of the PrlR or Omp89 was used to normalize total protein content.