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. 2013 Dec 11;8(12):e83005. doi: 10.1371/journal.pone.0083005

Figure 2. Expression of cardiac markers in R535Q-LQTS-3 iPS cell-derived CM.

Figure 2

(A) Immunocytochemical analysis of cardiac markers α-actinin (red) and troponin I (green). Nuclei were counterstained with DAPI (blue). Scale bars: 20 μm (α-actinin) and 100 μm (troponin I). (B) The analysis of expression levels of cardiospecific transcripts was done by RT-PCR showing that the R535Q-LQTS-3 iPS cell-derived cardiomyocytes express cardiac transcripts at a similar level as their human ES cell-derived counterparts.