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. 2013 Dec 11;8(12):e82827. doi: 10.1371/journal.pone.0082827

Figure 2. Over-expression of HIF-1α induces Mipu1 expression in H9C2 cells.

Figure 2

A: immunoblotting showing the expression of Mipu1 and HIF-1α mediated by pcDNA3.1-HIF-1α transfection (n=3). Upper panel showing the semi-quantitative analysis of HIF-1α and Mipu1 band against β-actin. * p<0.05 versus “Neo” (pcDNA3.1 vector), # p<0.05 versus “HIF” (HIF-1α transfection alone). B: Quantitative real time PCR showing the expression of mipu1 induced by HIF-1α transfection (n=3. triplicate for each sample), compared with “Neo”, * p<0.05. C: indirect immunofluorescence showing gene transfection-induced translocation of HIF-1α from cytoplasm to the nucleus (×400).