Figure 5. Mipu1 shRNA abolishes in part the cytoprotection of HIF-1α against oxidative stress in H9C2.

“Neg”: negative control shRNA vector (pRNA-u6.1), “shRNA”: Mipu1 shRNA expression plasmid (pRNA-u6.1- Mipu1), “N”: pcDNA3.1 vector (Neo), “H”: pcDNA3.1-HIF-1α vector. A: immunoblotting showing the effect of Mipu1 shRNA on expression of Mipu1 and HIF-1α; B: semi-quantitative analysis of HIF-1α and Mipu1 band against β-actin, * p<0.05 versus “N+Neg”, # p<0.05 versus “H+Neg”, (n=3); C: flowcytometry detecting the hypoploid cells induced by 0.5 mmol/L hydrogen peroxide (H₂O₂), * p<0.05 versus H₂O₂-untreated cells, # p<0.05 versus H₂O₂-treated “N” group, a p<0.05 versus responsive “Neg” group, (N=5). D: Colorimetric Assay showing the activity of Caspase-9 induced by H₂O₂ (n=4). * p<0.05 versus H₂O₂-untreated cells, # p<0.05 versus H₂O₂-treated “N” group, a p<0.05 versus responsive “Neg” group. E: LDH release rate, * p<0.05 versus H₂O₂-untreated cells, # p<0.05 versus H₂O₂-treated “N” group, a p<0.05 versus responsive “Neg” group, n=5.