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. 2013 Dec 11;8(12):e82585. doi: 10.1371/journal.pone.0082585

Figure 2. Requirement of heparin length for interaction with ECP.

Figure 2

(A) AMAC-labeled heparin oligosaccharide (10 nmol) were incubated with or without wtECP (heparin: wtECP ratio of 5) in PBS at 25 °C for 15 min, and the reaction products were separated by gel electrophoresis using 1% agarose gel. (B) Beas-2B cells were pre-incubated with heparin oligosaccharide in RPMI 1640 medium at 4 °C for 30 min before treatment with 0.4 μM of wtECP at 4 °C for an additional 1 h. The level of bound proteins was assayed by ELISA and the amount of wtECP bound to Beas-2B cells without heparin derivative treatment was set as 100%. C, untreated. C’, treated with EG2 antibody only. The data represented three independent experiments and the error bar was shown as SD. **, P < 0.01; ***, P < 0.001.