Figure 4. Putative heparin binding clamp in ECP with corresponding residues in EDN.

(A) Superimpose analysis was performed using Lsqkab. Hep6 bound pose of ECP was superimposed on that of EDN by fitting Cα atoms of residues 40 (Gln) and 64 (His/Ser). Distances between these two residues were analyzed by PyMOL. (B) Hep6 were shown in line and CPK color. Residues were shown in orange (ECP) and pink (EDN) sticks. Hydrogen bonds were shown in red dashed lines with indicated distance. The vDW forces between the corresponding residues on ECP/EDN and the surrounding sugar moieties with indicated moiety name and number were shown as curve colored in blue indicate. SGN, 6-O-sulfated, N-sulfated glucosamine; IDS, 2-O-sulfated iduronic acid.