Table 1. Competitive cellular binding effect of heparin derivatives.
Heparin derivative | Mean ± SD (%) |
|
---|---|---|
10 μg/ml | 50 μg/ml | |
Tetrasaccharide | 93.7 ± 10.9 | 86.7 ± 8.6 |
Hexasaccharide | 86.4 ± 6.9 | 75.4 ± 10.9 |
Octasaccharide | 54.5 ± 17.1 (**) | 39.2 ± 11.9 (***) |
Decasaccharide | 33.4 ± 5.6 (**) | 24.2 ± 3.5 (***) |
de-2-O-SH | 48.9 ± 8.6 (***) | 41.4 ± 13 (***) |
de-6-O-SH | 47 ± 8.5 (***) | 35.9 ± 8.7 (***) |
de-N-SH | 52.8 ± 4.5 (***) | 35.5 ± 12.6 (***) |
NAc-H | 62.1 ± 1.3 (***) | 55.5 ± 13.6 (***) |
NAc-de-O-SH | 95.8 ± 8.2 | 92.4 ± 9.8 |
HMWH | 56.8 ± 4.4 (***) | 28.9 ± 1.8 (***) |
LMWH | 42.6 ± 9.2 (***) | 33.2 ± 4 (***) |
The average amount of 0.4 μM wtECP bound to Beas-2B cells was set as 100% binding. **, P < 0.01; ***, P < 0.001