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. 2013 Dec 11;8(12):e83018. doi: 10.1371/journal.pone.0083018

Figure 4. BARD1 is the target of miR-19a and miR-19b.

Figure 4

(A) Real-Time PCR for mir-19a and miR-19b in NB4 cells transfected with mimic-miR-19a, mimic-miR-19b or mimic-miR-scramble (miR-s). Data show the mean value of three parallel experiments with error bars showing the standard deviations on top of each column. (B) BARD1 Real-Time PCR in NB4 cells transfected with mimic-miR-19a, mimic-miR-19b or mimic-miR-scramble. Data show the mean value of three parallel experiments with error bars showing the standard deviations on top of each column. (C) Luciferase assay in HeLa cells after transfection with 1 µg pGL3-3’UTR-BARD1 wild-type (left) and mutated (right) vectors plus mimic-miR-19a/b or scramble at a concentration of 200 nM. Data show the mean value of three parallel experiments with error bars showing the standard deviations on top of each column. Annealing of miR-19a and miR-19b to BARD1 3’UTR: wild type and mutated BARD1 3’UTR are schematized.