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A. Ectopic expression of Lin28 in pc-Lin28-1 and pc-Lin28-2 MCF-7 clones induced MCF-7 cells to adopt mesenchymal shapes. B and C. Western blotting (B) and Immunofluorescence staining (C) illustrated reduced expression of E-cadherin and increased expression of vimentin in pc-Lin28-1 and pc-Lin28-2 cells. E-cadherin: red, vimentin: green, cell nuclei: blue. Bar, 10 μm.
